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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
JoVE Journal
Genetica
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JoVE Journal Genetica
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

DOI:
10.3791/57831-v

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09:34 min

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October 25, 2018

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, , ,
1Division of Molecular Hematology, Lund Stem Cell Center,Lund University

Capitoli

  • 00:04Titolo
  • 00:50Lysis Plate Preparation and Cell Sorting
  • 03:35Reverse Transcription and Specific Target Amplification
  • 04:51Preparation of Sample and Assay Plates
  • 07:41Results: Dynamic Characterization of Cell Populations
  • 08:43Conclusion

Summary

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Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell populations.

Tags

Single-cell AnalysisStem Cell HeterogeneityCancer HeterogeneityImmunophenotypic ProfilingGene Expression AnalysisLineage PotentialFACS SortingSingle-cell PCRCombinatorial Approach

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