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酶联免疫吸附法表征小鼠胸腺依赖性和胸腺独立免疫球蛋白同种反应
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
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酶联免疫吸附法表征小鼠胸腺依赖性和胸腺独立免疫球蛋白同种反应

DOI:
10.3791/57843-v

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06:15 min

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September 07, 2018

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, ,
1Department of Cell Biology and Neuroscience and Graduate Program in Cellular and Molecular Pharmacology,Rutgers University, 2Department of Cell Biology and Neuroscience,Rutgers University, 3Rutgers Cancer Institute of New Jersey

Capitoli

  • 00:04Titolo
  • 00:432,4,6-Trinitrophenyl (TNP)-polysaccharide and TNP-keyhole Limpet Hemocyanin (KLH) Immunization and Serum Preparation
  • 01:54Enzyme-linked Immunosorbent Analysis (ELISA)
  • 04:17Results: Representative Thymus-independent (TI) and Thymus-dependent (TD) Ig Isotype responses to TNP-polysaccharide and TNP-KLH Immunization
  • 05:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文描述了一种用 ELISA 方法表征小鼠 t 依赖性和 t 无关免疫球蛋白 (同种) 反应的协议。这种方法单独使用或结合流式细胞术将允许研究人员确定在 B 细胞介导的小鼠同种反应在 t 依赖性和 t 独立抗原免疫的差异。

Tags

– Enzyme-linked Immunosorbent Assay (ELISA)- Immunoglobulin (Ig) Isotype- Thymus-dependent- Thymus-independent- B Cell Activation- Germinal Center- TNP-polysaccharide- Retro-orbital Bleeding- Ig Isotype-specific Capturing Antibodies- TNP-38BSA Antigen- TNP-3BSA Antigen- Blocking Buffer- Ig Isotype Standard

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