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一种基于变形虫的快速图像细菌毒力检测方法
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JoVE Journal Bioingegneria
A Rapid Image-based Bacterial Virulence Assay Using Amoeba
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一种基于变形虫的快速图像细菌毒力检测方法

DOI:
10.3791/57844-v

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05:22 min

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June 27, 2018

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,
1Department of Physics and Astronomy,University of California, 2Department of Molecular Biology and Biochemistry,University of California

Capitoli

  • 00:04Titolo
  • 00:46Growth and Maintenance of Amoebae
  • 01:57The Growth of P. aeruginosa
  • 02:16Preparation of Agar Pad, Preparation of Bacteria-amoeba Sample, and Image Acquisition
  • 04:05Results: Virulence of P. aeruginosa is Upregulated in Surface-attached Populations
  • 04:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个协议, 以测量浮游或表面附着细菌的毒力, 使用柄(变形虫) 作为宿主。在1小时内对毒力进行测量, 并使用荧光显微镜和图像分析对宿主杀死进行量化。我们用铜绿假单胞菌证明了这个协议。

Tags

Bacterial VirulenceAmoebaQuantitative AssayHost-killingSurface-attached BacteriaPlanktonic CellsPseudomonas AeruginosaE. ColiAgar PadCalcium AMDevelopment Buffer

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