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Vorbereitung von Drosophila Larven Proben für Gaschromatographie-Massenspektrometrie (GC-MS)-basierte Metabolomik
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics
DOI:

07:21 min

June 06, 2018

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Capitoli

  • 00:05Titolo
  • 00:37Larval Sample Collection
  • 01:28Sample Transfer to Bead Tubes
  • 02:23Sample Extraction
  • 03:52Chemical Derivatization
  • 06:20Results: Gas Chromatography-Mass Spectrometry Analyses Reveal Significant Differences Between Lactate Dehydrogenase Mutants and Wild-type Controls
  • 07:02Conclusion

Summary

Traduzione automatica

Dieses Protokoll beschreibt die Drosophila Larven für GC-MS-basierte Metabolomic Analyse vorzubereiten.

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