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Capitoli
- 00:05Titolo
- 00:37Guide RNA Design
- 03:37Guide RNA Cloning
- 06:27Transfection of Enhancer-I, Cell Harvest, and RNA Extraction
- 08:21Results: Guide RNA Design/Cloning and Evaluation of Gene Expression in Response to Enhancer-i
- 09:23Conclusion
This protocol describes the steps needed to design and perform multiplexed targeting of enhancers with the deactivating fusion protein SID4X-dCas9-KRAB, also known as enhancer interference (Enhancer-i). This protocol enables the identification of enhancers that regulate gene expression and facilitates the dissection of relationships between enhancers regulating a common target gene.
