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Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion
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Neuroscienze
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JoVE Journal Neuroscienze
Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

DOI:
10.3791/57894-v

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05:58 min

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July 04, 2018

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, ,
1Department of Reproductive Medicine,University of California, San Diego School of Medicine

Capitoli

  • 00:04Titolo
  • 00:49Acclimatization to Handling
  • 02:12Blood Collection
  • 04:26Results: Luteinizing Hormone Dynamics in Response to Acute Psychosocial Stres
  • 05:19Conclusion

Summary

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Here we present a tail-tip blood sampling protocol for frequent sample collection in unrestrained mice. This method is useful for assessing patterns of pulsatile luteinizing hormone secretion and could be adapted for analysis of other circulating factors.

Tags

Tail-tip Blood SamplingLuteinizing Hormone SecretionPulsatile SecretionReproductive NeuroendocrinologyMouse HandlingBlood CollectionACES BufferBio-safety Cabinet

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