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A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue
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Capitoli
- 00:04Titolo
- 00:34Sandwiching Cell Sheet Seeding and SWAT Supply Preparation
- 02:02Gelatin Plunger Preparation
- 03:40White Adipose Tissue Processing
- 05:49Results: Representative SWAT Analyses
- 07:23Conclusion
White adipose tissue (WAT) has critical deficiencies in its current primary culture models, hindering pharmacological development and metabolic studies. Here, we present a protocol to produce an adipose microphysiological system by sandwiching WAT between sheets of stromal cells. This construct provides a stable and adaptable platform for primary WAT culture.
