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Påvisning af Protein subcellulært lokalisering af grøn fluorescens Protein Tagging og 4', 6-Diamidino-2-phenylindole farvning i Caenorhabditis elegans
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Biologia
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JoVE Journal Biologia
Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4′,6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Påvisning af Protein subcellulært lokalisering af grøn fluorescens Protein Tagging og 4', 6-Diamidino-2-phenylindole farvning i Caenorhabditis elegans

DOI:
10.3791/57914-v

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09:36 min

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July 30, 2018

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, ,
1Department of Science,Borough of Manhattan Community College/CUNY

Capitoli

  • 00:04Titolo
  • 00:26Heat Stress
  • 02:46Ethanol Fixation and DAPI Staining
  • 04:10Acetone Fixation
  • 05:21Microscopic Imaging
  • 07:56Results: Visualization of C. elegans Exl-1 Subcellular Localization through GFP Tagging and DAPI Staining
  • 09:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol demonstrerer, hvordan man spore en protein nukleare translokation under varmestress ved hjælp af en grøn fluorescens protein (NGL) fusion protein som en markør og 4', 6-diamidino-2-phenylindole (DAPI) farvning. DAPI farvning protokol er hurtig og bevarer normal god landbrugspraksis og protein subcellulært lokalisering signaler.

Tags

Protein Subcellular LocalizationGFP TaggingDAPI StainingCaenorhabditis ElegansCell BiologyTranslational ConstructExtrachromosomal ArrayGamma RadiationTransgenic LineStress AssayHeat StressM9 BufferSynchronizationL4 Larvae

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