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o 型血管哺乳动物细胞产生均匀集料的轨道晃动培养

JoVE Journal
Bioingegneria

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JoVE Journal Bioingegneria

An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates

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o 型血管哺乳动物细胞产生均匀集料的轨道晃动培养

DOI:

10.3791/57922-v

•

05:40 min

•

January 07, 2019

•

Ikki Horiguchi², Ikumi Suzuki, Takashi Morimura, Yasuyuki Sakai⁴

¹Department of Chemical System Engineering,University of Tokyo, ²Department of Biotechnology,Osaka University, ³Biotech Business Unit,Fukoku Co. Ltd, ⁴Institute of Industrial Science,University of Tokyo

Capitoli

00:04Titolo
01:18Seeding Cells in an O-shaped Bag
02:42Medium Change
03:11Cell Collection
04:07Results: Morphology and Viability of Aggregates
05:18Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个使用 o 型容器的协议, 专门用于悬浮培养的细胞集料, 轨道晃动。在这个袋子里生长的 hek293 细胞形成比传统培养容器中生长的细胞更均匀的聚集物。

Tags

Keywords: Orbital Shaking Culture Mammalian Cells O-shaped Vessels Uniform Aggregates Drug Screening Suspension Culture HEK-293 Cells Cell Aggregation Cell Culture Bioreactor Design

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