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成像 FITC-葡聚糖作为胞吐作用调节的记者
JoVE Journal
Biologia
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JoVE Journal Biologia
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
DOI:

04:50 min

June 20, 2018

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Capitoli

  • 00:04Titolo
  • 00:34Preparations and Culture of RBL Cells for Live Cell Microscopy
  • 01:52Live Cell Microscopy of Exocytosis
  • 03:43Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
  • 04:17Conclusion

Summary

Traduzione automatica

在这里, 我们详细介绍了一种带电细胞成像的调节胞吐作用方法。该方法利用 FITC-葡聚糖, 在溶酶体相关细胞器中积累, 作为记者。这一简单的方法也允许区分不同模式的调节胞吐作用在细胞难以操纵基因。

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