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Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies
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Biologia
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JoVE Journal Biologia
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies

Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies

DOI:
10.3791/57944-v

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12:42 min

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July 14, 2018

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, , , , , , , ,
1Centre for Experimental Medicine,Queen’s University of Belfast, 2Centre for Biomedical Sciences (Education),Queen’s University of Belfast, 3Department of Pharmaceutical Chemistry and Pharmacognosy,Naresuan University, 4School of Medicine, Dentistry and Biomedical Sciences,Queen’s University of Belfast

Capitoli

  • 00:00Titolo
  • 01:23Isolation of Retinal Arterioles
  • 05:09Arteriolar Pressure Myography
  • 07:22Patch-Clamp Electrophysiology
  • 09:58Risultati
  • 12:00Conclusion

Summary

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This manuscript describes a straightforward protocol for the isolation of arterioles from the rat retina that can be used in electrophysiological, calcium imaging and pressure myography studies.

Tags

Retinal ArteriolesRetinal Blood FlowRetinal PerfusionVascular Smooth MusclePatch ClampCalcium ImagingPressure MyographyRetinal DiseasesDiabetic RetinopathyGlaucomaBranch Vein OcclusionsEye DissectionRetina IsolationLow Calcium Hanks’ Solution

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