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Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction
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JoVE Journal Medicina
Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

DOI:
10.3791/57953-v

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08:31 min

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July 26, 2018

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, , , ,
1Department of Internal Medicine and Cardiology,Charité University Medicine, 2German Center for Cardiovascular Research (DZHK)

Capitoli

  • 00:04Titolo
  • 00:33Preparation of the Rat Heart
  • 01:35Cannulation
  • 02:50Pressure Manipulation and Digestion
  • 04:25Cell Processing and Calcium Re-adaptation
  • 05:57Functional Evaluation of Excitation-contraction-coupling
  • 07:32Results: Staining of Rat ACMs with Fluo4-AM
  • 08:02Conclusion

Summary

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Here, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes from a rat model of metabolic syndrome-related heart failure with preserved ejection fraction. A manual regulation of intraluminal pressure of cardiac cavities is implemented to yield functionally intact myocytes suitable for excitation-contraction-coupling studies.

Tags

Keywords: Atrial CardiomyocytesRat ModelMetabolic SyndromeHeart FailurePreserved Ejection FractionLangendorff ApparatusCannulationCardiomyocyte IsolationExcitation-contraction-coupling

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