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En temperaturgradient Assay til at fastlægge termisk præferencer af Drosophila larver
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Neuroscienze
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JoVE Journal Neuroscienze
A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

En temperaturgradient Assay til at fastlægge termisk præferencer af Drosophila larver

DOI:
10.3791/57963-v

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08:59 min

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June 25, 2018

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, ,
1Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology,University of California, Santa Barbara, 2Division of Cell Signaling, National Institute for Physiological Sciences,National Institutes of Natural Sciences, 3Thermal Biology Group, Exploratory Research Center on Life and Living Systems,National Institutes of Natural Sciences

Capitoli

  • 00:05Titolo
  • 00:43Larval Synchronization
  • 02:03Single-directional Temperature Gradient Setup
  • 03:30Larval Collection and Washing
  • 05:08Assay and Calculation
  • 06:25Results: Larvae Distribution from 18 °C – 28 °C
  • 08:13Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi præsenterer her, en protokol for at bestemme den foretrukne miljømæssige temperaturen i Drosophila larver ved hjælp af en kontinuerlig termisk gradient.

Tags

Keywords: Temperature GradientThermal PreferenceDrosophila LarvaeSomatosensationTemperature AssayC. ElegansYeast PasteEgg CollectionAgarose PlateAluminum BlocksWater Bath

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