Rivista
/
Biologia dello sviluppo
/
多能干细胞半自动化生产肝样细胞的研究
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells
Please note that all translations are automatically generated. Click here for the English version.

多能干细胞半自动化生产肝样细胞的研究

DOI:
10.3791/57995-v

•

05:43 min

•

July 27, 2018

•

, , ,
1MRC Centre for Regenerative Medicine,University of Edinburgh

Capitoli

  • 00:04Titolo
  • 00:30Seeding Human Pluripotent Stem Cells (hPSCs)
  • 02:32Differentiating hPSCs on Recombinant Laminins and Characterization
  • 04:01Results: Verification of Successful Production via Hepatocyte Markers
  • 04:58Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了一种半自动化的方法, 从96井板格式的人类多潜能干细胞中产生肝样细胞。这一过程是快速和成本效益, 允许生产质量保证批次的肝细胞样干细胞的基础和应用人类研究。

Tags

Keywords: Semi-automatedHepatocyte-like CellsPluripotent Stem CellsLaminin 521MTeSR1 MediumROCK Inhibitor Y27632Definitive Endoderm InductionKSR DMSO MediumHepatoblast SpecificationHepatoZYME Medium96-well FormatAutomated Liquid HandlingMass ProductionCell ConfluencyDifferentiation

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/57995?article_type=v

从人多能干细胞的黑素细胞的无饲养推导
check_url/it/57995?article_type=v

诱导多能干细胞使用成纤维样滑膜生成类风湿关节炎患者的关节分离
check_url/it/57995?article_type=v

从人多能干细胞使用高度可重复的小分子为基础的分化协议心肌细胞规模化生产
check_url/it/57995?article_type=v

从小鼠羊水细胞多能干细胞使用转座子系统的生产
check_url/it/57995?article_type=v

界定和由人多能干细胞的肝细胞样细胞的可扩展代
check_url/it/57995?article_type=v

水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案
check_url/it/57995?article_type=v

活完好斑马鱼的单细胞 Photoconversion
check_url/it/57995?article_type=v

人诱导多潜能干细胞衍生肝细胞的药物发现方法
check_url/it/57995?article_type=v

人多潜能干细胞第一心场样心脏祖细胞和心室样细胞的生成
check_url/it/57995?article_type=v

秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布

Read Article