Rivista
/
Biochimica
/
台式固定化金属亲和层析、Polyhistidine 标记金属酶的重组与检测
JoVE Journal
Biochimica
This content is Free Access.
JoVE Journal Biochimica
Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
Please note that all translations are automatically generated. Click here for the English version.

台式固定化金属亲和层析、Polyhistidine 标记金属酶的重组与检测

DOI:
10.3791/58012-v

•

08:02 min

•

August 23, 2018

•

,
1Department of Chemistry,Muhlenberg College

Capitoli

  • 00:04Titolo
  • 00:34Preparation of the IMAC Column
  • 01:42Purification of Polyhistidine-tagged Target by IMAC
  • 04:32Reconstitution of Target Protein with Fe2+
  • 06:43Results: Undergraduate Performed Assay of a Polyhistidine Tagged Metalloenzyme
  • 07:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个协议, 台式固定化金属亲和层析纯化和随后重组的 polyhistidine 标签, 非血红素铁结合酶适合本科教学实验室。

Tags

Benchtop Immobilized Metal Affinity ChromatographyPolyhistidine Tagged MetalloenzymeUndergraduate LaboratoryNon-heme Iron-binding EnzymesNickel-bound Nitriloacetic Acid ResinLysis Bind BufferCell-free Crude ExtractsFlow-throughEluent

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/58012?article_type=v

从Cryomilled哺乳动物细胞中的蛋白质复合物亲和捕获
check_url/it/58012?article_type=v

细菌显示文库的半自动化筛选肽亲和试剂的发现与分离结果分析
check_url/it/58012?article_type=v

蛋白质消化、超滤和尺寸排除色谱法优选人血浆和血清外来体的分离
check_url/it/58012?article_type=v

毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析
check_url/it/58012?article_type=v

光谱分析 TRPV1 的纯化与重构
check_url/it/58012?article_type=v

丝状真菌曲霉菌的单步富集蛋白脱乙酰化酶的酶活性测定
check_url/it/58012?article_type=v

用于研究拉帕霉素/mTOR相互作用的半定量药物亲和反应目标稳定性 （DARTS） 测定
check_url/it/58012?article_type=v

洗涤剂辅助重组重组果蝇阿拉他素到脂质混合测定脂质体
check_url/it/58012?article_type=v

活联甘蔗，用于亲和色谱树脂的快速发展 - 抗体捕获作为案例研究
check_url/it/58012?article_type=v

用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应

Read Article