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Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
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Biochimica
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JoVE Journal Biochimica
Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids

Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids

DOI:
10.3791/58017-v

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11:28 min

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August 28, 2018

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, ,
1Institute for Molecular Biosciences,Goethe University

Capitoli

  • 00:04Titolo
  • 00:34Thylakoid Isolation from C. meneghiniana Cells
  • 03:13Separation and Concentration of FCP Complexes
  • 05:22Preparation of Liposomes and Incorporation of FCP
  • 07:18Incorporation of FCP Complexes and Removal of Detergents and Aggregates
  • 09:59Results: Spectral Features of FCP-liposomes in Response to Ion Gradients
  • 11:07Conclusion

Summary

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Here, we present a protocol to isolate fucoxanthin chlorophyll a/c binding proteins (FCP) from diatoms and incorporate them into liposomes with natural lipid compositions to study excitation energy transfer upon ion composition changes.

Tags

Diatom Cyclotella MeneghinianaLight-harvesting AntennasLiposomesThylakoid LipidsPhotosynthesis ResearchMembrane CompartmentIon And PH ChangesCell DisruptionThylakoid MembranesSucrose Gradient

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