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研究核苷酸合并: 酵母基因组 Ribonucleotides 的链特异检测及核苷酸诱变的检测
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Biologia
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JoVE Journal Biologia
Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
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研究核苷酸合并: 酵母基因组 Ribonucleotides 的链特异检测及核苷酸诱变的检测

DOI:
10.3791/58020-v

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09:04 min

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July 26, 2018

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, ,
1Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, NIH, DHHS

Capitoli

  • 00:04Titolo
  • 00:49Alkaline Hydrolysis of Genomic DNA
  • 02:36Strand specific Southern Blot: Transfer
  • 04:37Strand-specific Southern Blot: Hybridization
  • 06:48Results: Assessment of Ribonucleotide Incorporation into DNA
  • 08:08Conclusion

Summary

Traduzione automatica

Traduzione automatica

Ribonucleotides 是在核 DNA 复制过程中纳入基因组的最丰富的非规范核苷酸之一。如果没有正确去除, ribonucleotides 会导致 DNA 损伤和突变。在这里, 我们提出了两个实验方法, 用于评估核苷酸的丰度纳入 DNA 及其诱变效应。

Tags

Keywords: Ribonucleotide IncorporationStrand-specific DetectionYeast GenomeRibonucleotide-induced MutagenesisAlkaline Gel ElectrophoresisSouthern BlottingGenomic DNAPotassium HydroxideAlkaline DNA Loading BufferAgarose GelSodium HydroxideEDTADNA Size MarkerNeutralization BufferSYBR Gold StainUV TransilluminatorAlkaline Transfer BufferNylon MembraneCapillary Action

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