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The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression
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JoVE Journal Genetica
The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

DOI:
10.3791/58022-v

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06:50 min

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July 24, 2018

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1Department of Biology,University of Hartford

Capitoli

  • 00:04Titolo
  • 00:31Media Preparation, Splenocyte Isolation, and Challenge
  • 03:02RNA Isolation and cDNA Synthesis
  • 04:50Quantitative Polymerase Chain Reaction (qPCR)
  • 05:40Results: Clock Gene Expression
  • 06:33Conclusion

Summary

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This protocol describes a technique using mouse splenocytes to discover pathogen-associated molecular patterns that alter molecular clock gene expression.

Tags

Mouse SplenocytesPathogen-associated Molecular PatternClock Gene ExpressionImmunologyChronobiologyPAMPCell CultureCell HomogenizationCell CountingCell StimulationRNA Extraction

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