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Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
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JoVE Journal Neuroscienze
Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

DOI:
10.3791/58028-v

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07:33 min

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December 11, 2018

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, , ,
1Laboratory of Neurobiology, Neuroscience Program, Bellvitge Biomedical Research Institute (IDIBELL),L’Hospitalet de Llobregat, 2Department of Pathology and Experimental Therapy, School of Medicine, University of Barcelona,L’Hospitalet de Llobregat, 3Institute of Neurosciences, University of Barcelona,L’Hospitalet de Llobregat

Capitoli

  • 00:04Titolo
  • 00:34Transection of Olfactory Nerves
  • 01:34Labeling of Olfactory Receptor Neurons with Fluorescent Calcium Indicators
  • 02:29Preparation of Tadpoles for Live Imaging of Presynaptic Responses
  • 04:09Live Imaging of Presynaptic Ca2+ Changes in Olfactory Glomeruli
  • 04:47Olfactory-guided Behavior Assay
  • 05:43Results: Tracking Olfactory-guided Behavior
  • 07:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

Xenopus tadpoles offer a unique platform to investigate the function of the nervous system in vivo. We describe methodologies to evaluate the processing of olfactory information in living Xenopus larvae in normal rearing conditions or after injury.

Tags

Olfactory PathwaysXenopus TadpolesFunctional EvaluationNeurobiologyPresynaptic TerminalsIn VivoCalcium GreenOlfactory BulbAnesthetizationTransectionBehavioral ExperimentsImaging

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