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甲苯胺蓝染色树脂嵌段评价周围神经形态学

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology

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甲苯胺蓝染色树脂嵌段评价周围神经形态学

DOI:

10.3791/58031-v

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08:17 min

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July 03, 2018

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Adel B. Ghnenis, Richard E. Czaikowski, Zhaojie J. Zhang, Jared S. Bushman

¹School of Pharmacy,University of Wyoming, ²Department of Zoology and Physiology,University of Wyoming

Capitoli

00:04Titolo
00:36Surgery and In Vivo Nerve Fixation
02:32Osmium Tetroxide Treatment and Resin Embedding
04:38Sectioning by Ultramicrotome
05:58Toluidine Blue Staining
06:47Results: Transverse Section Images of a Rat Sciatic Nerve Stained with Toluidine Blue
07:48Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个通过获取和染色1-2 µm 段与甲苯胺蓝可视化周围神经精细结构的协议

Tags

Toluidine Blue Staining Resin-embedded Sections Peripheral Nerve Morphology Nerve Injury Nerve Regeneration Sciatic Nerve Nerve Fixation Nerve Segmentation Osmium Tetroxide Epoxy Embedding

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