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Biologie cellulaire quantitative de neurodégénération chez la drosophile grâce à l’analyse impartiale des protéines fluorescent étiquetées à l’aide de ImageJ
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1Department of Molecular and Cellular Pharmacology,University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Yantai University
Capitoli
- 00:04Titolo
- 00:50Brain Dissection and Immunofluorescence Staining
- 02:23Image Acquisition and Region of Interest (ROI) Selection
- 04:19Preprocessing and Segmentation with H-Maxima Watershedding and Quantity, Area, and Intensity Quantification
- 06:30Results: Representative Quantitative Analysis of Cell Biology of Neurodegeneration
- 08:20Conclusion
Nous avons développé un workflow simple et adaptable pour extraire des données quantitatives d’études biologiques de fluorescence dotés d’imagerie cellulaire d’agrégation des protéines et du flux autophagie dans le système nerveux central de Drosophila modèles de neurodégénérescence.
Tags
Quantitative Cell BiologyNeurodegenerationDrosophilaFluorescently Tagged ProteinsImageJImage Analysis WorkflowProteinaceous PunctaMembrane-bound CompartmentsNeurodegenerative DiseasesLamina DissectionRetina RemovalBrain DissectionImmunostainingAntibody IncubationMountingMicroscopyFluorescence Imaging
