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Neuroscience

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JoVE Journal Neuroscience
An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins
 

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

Article DOI: 10.3791/58043-v • 09:33 min • June 26th, 2018
June 26th, 2018 •
1, 1, 1, 1
1Institute for Anatomy and Embryology, University Medical Centre Göttingen

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We describe an optical assay for synaptic vesicle (SV) recycling in cultured neurons. Combining this protocol with double transfection to express a presynaptic marker and protein of interest allows us to locate presynaptic sites, their synaptic vesicle recycling capacity, and determine the role of the protein of interest.

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Keywords: Optical Assay Synaptic Vesicle Recycling Cultured Neurons Presynaptic Proteins Antibody Specificity Primary Hippocampal Cells Transfection Calcium Chloride Endotoxin-free DNA Transfection Buffer Reduced-serum Medium Cell-culture Medium Incubation Washing Active Synapses
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