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The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
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JoVE Journal Biochimica
The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

DOI:
10.3791/58059-v

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09:35 min

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July 17, 2018

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, ,
1Department of Fisheries and Wildlife,Michigan State University

Capitoli

  • 00:04Titolo
  • 00:27Collection and Extraction of Sea Lamprey Conditioned Water
  • 01:14Isolation of Fraction Pools with Chromatography
  • 02:51Electro-olfactogram Recordings to Identify Odorous Fractions/Compounds
  • 05:43Two-choice Maze Behavioral Bioassay to Identify Behaviorally Active Fractions/Compounds
  • 06:31Chromatographic Isolation of Pure Compounds from Active Fractions
  • 06:58Structure Elucidation of a Pure Compound with Mass Spectrometry and Nuclear Magnetic Resonance
  • 07:58Results: Behavioral Responses of Ovulated Female Sea Lampreys to Odorants
  • 08:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to isolate and characterize the structure, olfactory potency, and behavioral response of putative pheromone compounds of sea lampreys.

Tags

Keywords: Sea LampreyPheromone IdentificationBioassay-guided FractionationChemical EcologySolid-phase ExtractionSilica Gel ChromatographyThin-layer ChromatographyElectro-olfactogram Recording

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