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Die Identifizierung von Meerneunauge Pheromone mit Bioassay-geführte Fraktionierung
JoVE Journal
Biochimica
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JoVE Journal Biochimica
The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
DOI:

09:35 min

July 17, 2018

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Capitoli

  • 00:04Titolo
  • 00:27Collection and Extraction of Sea Lamprey Conditioned Water
  • 01:14Isolation of Fraction Pools with Chromatography
  • 02:51Electro-olfactogram Recordings to Identify Odorous Fractions/Compounds
  • 05:43Two-choice Maze Behavioral Bioassay to Identify Behaviorally Active Fractions/Compounds
  • 06:31Chromatographic Isolation of Pure Compounds from Active Fractions
  • 06:58Structure Elucidation of a Pure Compound with Mass Spectrometry and Nuclear Magnetic Resonance
  • 07:58Results: Behavioral Responses of Ovulated Female Sea Lampreys to Odorants
  • 08:36Conclusion

Summary

Traduzione automatica

Hier präsentieren wir Ihnen ein Protokoll zu isolieren und zu charakterisieren, Struktur, olfaktorische Potenz und Verhaltensreaktion des vermeintlichen Pheromon Verbindungen der Meer Neunaugen.

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