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Identifiering av havet nejonöga feromoner med hjälp av Bioassay-guidad fraktionering
JoVE Journal
Biochimica
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JoVE Journal Biochimica
The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
DOI:

09:35 min

July 17, 2018

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Capitoli

  • 00:04Titolo
  • 00:27Collection and Extraction of Sea Lamprey Conditioned Water
  • 01:14Isolation of Fraction Pools with Chromatography
  • 02:51Electro-olfactogram Recordings to Identify Odorous Fractions/Compounds
  • 05:43Two-choice Maze Behavioral Bioassay to Identify Behaviorally Active Fractions/Compounds
  • 06:31Chromatographic Isolation of Pure Compounds from Active Fractions
  • 06:58Structure Elucidation of a Pure Compound with Mass Spectrometry and Nuclear Magnetic Resonance
  • 07:58Results: Behavioral Responses of Ovulated Female Sea Lampreys to Odorants
  • 08:36Conclusion

Summary

Traduzione automatica

Här presenterar vi ett protokoll för att isolera och karakterisera strukturen, lukt potens och beteendemässiga svar av förmodad pheromone föreningar av havet nejonöga.

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