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Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing
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Medicina
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JoVE Journal Medicina
Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

DOI:
10.3791/58071-v

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07:28 min

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July 10, 2018

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, ,
1Helsinki Institute of Life Science, Institute of Biotechnology,University of Helsinki, 2School of Medicine and Institute for Science and Technology in Medicine,Keele University

Capitoli

  • 00:04Titolo
  • 00:52Preparation
  • 01:41Corneal Abrasion
  • 02:46Imaging the Abrasion
  • 03:46Post Corneal Abrasion Procedures
  • 04:18Cornea Collection
  • 05:10Results: Detection and Localization of the Corneal Abrasion
  • 06:49Conclusion

Summary

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This protocol describes a method to inflict an abrasion to the ocular surface of the mouse, and to follow the wound healing process thereafter. The protocol takes advantage of an ocular burr to partially remove the surface epithelium of the eye in anaesthetized mice.

Tags

Corneal Epithelial AbrasionOcular BurrCornea Wound HealingCorneal Epithelial Progenitor CellsFluoresceinMouse ModelAnesthesiaCorneal Abrasion Technique

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