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高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

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高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究

DOI:

10.3791/58074-v

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07:22 min

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August 14, 2018

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Anthony M. Esposito², Alexandra Y. Soare, Foramben Patel, Namita Satija, Benjamin K. Chen, Talia H. Swartz

¹Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, ²Department of Biology,New Jersey City University

Capitoli

00:04Titolo
00:54Preparation of Target Cells for Cell-to-Cell Virus Transmission
02:11Preparation of Donor Cells
03:56Co-culture
05:06Fluorescence-activated Cell Sorting
05:35Results: Identification of a Novel HIV-1 Viral Membrane Fusion Inhibitor
06:49Conclusion

Summary

Traduzione automatica

通过流式细胞术或荧光显微镜检测绿色荧光蛋白的表达, 我们描述了以细胞为基础的 HIV-1 融合报告。它可以用来测试病毒进入的抑制剂 (特别是在融合步骤) 在无细胞和细胞对细胞感染系统。

Tags

Keywords: Cre-Lox Viral Membrane Fusion HIV-1 Drug Discovery High-throughput Screening HIV Fusion Inhibitors Biosafety Jurkat RG Reporter Cells Puromycin Cell-to-cell Virus Transmission Gag-iCre DNA Electroporation Ficoll

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