Rivista
/
Immunologia e infezioni
/
En høj overførselshastighed Cre-Lox aktiveret Viral membran Fusion analyse for at identificere hæmmere af HIV-1 Viral membran Fusion
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

En høj overførselshastighed Cre-Lox aktiveret Viral membran Fusion analyse for at identificere hæmmere af HIV-1 Viral membran Fusion

DOI:
10.3791/58074-v

•

07:22 min

•

August 14, 2018

•

, , , , ,
1Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology,New Jersey City University

Capitoli

  • 00:04Titolo
  • 00:54Preparation of Target Cells for Cell-to-Cell Virus Transmission
  • 02:11Preparation of Donor Cells
  • 03:56Co-culture
  • 05:06Fluorescence-activated Cell Sorting
  • 05:35Results: Identification of a Novel HIV-1 Viral Membrane Fusion Inhibitor
  • 06:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi beskriver en celle-baseret analyse for at rapportere om HIV-1 fusion via udtryk for grøn fluorescerende proteiner kan påvises ved flow flowcytometri eller Fluorescens mikroskopi. Det kan bruges til at teste hæmmere af viral entry (specielt på trinnet fusion) i celle-fri og celle til celle infektion systemer.

Tags

Keywords: Cre-LoxViral Membrane FusionHIV-1Drug DiscoveryHigh-throughput ScreeningHIV Fusion InhibitorsBiosafetyJurkat RG Reporter CellsPuromycinCell-to-cell Virus TransmissionGag-iCre DNAElectroporationFicoll

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/58074?article_type=v

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
check_url/it/58074?article_type=v

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
check_url/it/58074?article_type=v

Etablering af en Liquid-dækket kultur Polarized humane luftveje Epitelial Calu-3-celler til at studere Host celle respons på luftvejspatogener<em> In vitro</em
check_url/it/58074?article_type=v

High-throughput Screening for Bredspektret Chemical hæmmere af RNA virus
check_url/it/58074?article_type=v

Konformationel Evaluering af HIV-1 trimere kappeglycoproteinerne hjælp af en celle-ELISA-assay
check_url/it/58074?article_type=v

Nucleocapsid Annealing-medieret Electrophoresis (NAME) Indhold Tillader hurtig identifikation af HIV-1 nucleocapsid inhibitorer
check_url/it/58074?article_type=v

Fag Phenomics: Fysiologiske tilgange til Karakterisere Novel virale proteiner
check_url/it/58074?article_type=v

Udviklingen af ​​Frysetørret Loop-medierede Isotermiske Amplification reagenser til påvisning af<em> Coxiella burnetii</em
check_url/it/58074?article_type=v

Måling Influenza neuraminidase Inhibering Antistoftitere af Enzyme-linked lectin Assay
check_url/it/58074?article_type=v

Swab Sampling metode til påvisning af menneskelige Norovirus på overflader

Read Article