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Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages
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Biologia
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JoVE Journal Biologia
Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

DOI:
10.3791/58081-v

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09:07 min

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October 30, 2018

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, , ,
1Laboratory of Leukocyte Signalling,Institute of Molecular Genetics of the ASCR, 2Faculty of Science,Charles University, 3Department of Biophysical Chemistry,J. Heyrovsky Institute of Physical Chemistry ASCR

Capitoli

  • 00:04Titolo
  • 00:40Retrovirus Production
  • 02:38Murine Bone Marrow Cell Isolation and Macrophage Differentiation
  • 05:29Bone Marrow-derived Macrophage (BMDM) and Dendritic Cell (BMDC) EGFP-dagged Protein of Interest Production
  • 06:53Results: Representative Fluorescent Fusion Protein Expression in Murine BMDM and BMDC
  • 07:52Conclusion

Summary

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In this article, we provide a detailed protocol for the expression of fluorescent fusion proteins in murine bone marrow derived dendritic cells and macrophages. The method is based on the transduction of bone marrow progenitors with retroviral constructs followed by differentiation into macrophages and dendritic cells in vitro.

Tags

Fluorescent Fusion ProteinsMurine Bone Marrow-derived Dendritic CellsMacrophagesRetroviral TransductionPlatinum Eco-packaging CellsEcotropic Retroviral ParticlesBone Marrow Harvesting

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