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Isolation and Identification of Extravascular Immune Cells of the Heart
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Capitoli
- 00:04Titolo
- 00:40Circulating Leukocyte Labeling and Analysis
- 06:22Results: Cardiac Antigen Presenting Cell Quantification
- 07:53Conclusion
This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.
