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心脏外免疫细胞的分离与鉴定
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Identification of Extravascular Immune Cells of the Heart
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心脏外免疫细胞的分离与鉴定

DOI:
10.3791/58114-v

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08:24 min

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August 23, 2018

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, ,
1Toronto General Hospital Research Institute,University Health Network (UHN), 2Dept of Laboratory Medicine and Pathobiology,University of Toronto, 3Dept of Immunology,University of Toronto, 4Peter Munk Cardiac Centre, 5Ted Rogers Centre for Heart Research

Capitoli

  • 00:04Titolo
  • 00:40Circulating Leukocyte Labeling and Analysis
  • 06:22Results: Cardiac Antigen Presenting Cell Quantification
  • 07:53Conclusion

Summary

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该协议提出了一种简便、有效的方法, 用于分离、鉴定和量化在小鼠心肌中的免疫细胞在稳态或炎症状态下的存在。该协议结合酶和机械消化的生成单细胞悬浮, 可以进一步分析的流式细胞术。

Tags

Immune CellsHeartImmunophenotypingCardiac Immune Cell PopulationsTail Vein InjectionFluorophore-conjugated Anti-CD45 AntibodyHeart PerfusionIntravascular Contaminants

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