Rivista
/
Biochimica
/
Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

DOI:
10.3791/58117-v

•

05:08 min

•

August 24, 2018

•

, , , , ,
15th Medical Department, Medical Faculty Mannheim,Heidelberg University, 2Department of Internal Medicine,Heidelberg University, 3German Center for Diabetes Research (DZD), 4European Center for Angioscience, Medical Faculty Mannheim,Heidelberg University

Capitoli

  • 00:04Titolo
  • 00:27Large Scale Culture on Plates
  • 01:15Transfer of Large Amounts of Caenorhabditis elegans
  • 01:52Harvest of a Large Caenorhabditis elegans Sample
  • 03:20Protein Isolation for Mass Spectrometry
  • 03:56Results: Reproducible Quantitative Protein Isolation and Further Applications of Large-scale C. elegans Culture
  • 04:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes a method for the large-scale cultivation of Caenorhabditis elegans on solid media. As an alternative to liquid culture, this protocol allows obtaining parameters of different scales under plate-based cultivation. This increases the comparability of results by omitting the morphological and metabolic differences between liquid and solid media culture.

Tags

Caenorhabditis ElegansPlate-based CultivationLarge-scale CultivationNematode PopulationSynchronized PopulationM9 BufferSample PreparationMetabolic AlterationsDiabetesMicroscopeDensity EvaluationPipet TipsShear StressLiquid NitrogenCentrifugationSample WashingSnap Freezing

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Read Article