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Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes
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Biologia
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JoVE Journal Biologia
Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

DOI:
10.3791/58134-v

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06:59 min

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September 27, 2018

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, , , , ,
1Medical Department I, University Hospital Klinikum rechts der Isar,Technical University of Munich, 2German Centre for Cardiovascular Research (DZHK), Munich Heart Alliance, 3Beth Israel Deaconess Medical Center,Harvard Medical School

Capitoli

  • 00:04Titolo
  • 00:50Induced Pluripotent Stem Cell (iPS Cell)-derived Cardiomyocyte Preparation and Optical Membrane Potential Recording
  • 03:25Analysis
  • 04:55Results: Representative Optical Action Potential Measurement Analyses
  • 06:36Conclusion

Summary

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Here we present a method to optically image action potentials, specifically in ventricular-like induced pluripotent stem cell-derived cardiomyocytes. The method is based on the promoter-driven expression of a voltage-sensitive fluorescent protein.

Tags

Keywords: Human Induced Pluripotent Stem Cell-derived Ventricular CardiomyocytesOptical Action Potential RecordingsGenetically Encoded Voltage SensorsLentiviral TransductionCardiomyocyte Maintenance MediumTyrode’s SolutionEpifluorescence MicroscopeImage SplitterElectrical PacingStimulus GeneratorHigh-speed Imaging

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