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体外支持性脂质双层自组织蛋白模式的重组
JoVE Journal
Biochimica
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JoVE Journal Biochimica
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
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体外支持性脂质双层自组织蛋白模式的重组

DOI:
10.3791/58139-v

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08:10 min

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July 28, 2018

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, ,
1Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry

Capitoli

  • 00:04Titolo
  • 00:24Small Unilamellar Vesicle (SUV) Preparation
  • 01:20Cleaning Glass Coverslips
  • 02:13Chamber Assembly and Supported Lipid Bilayer (SLB) Formation
  • 04:15PDMS microstructures
  • 05:48Self-organization in PDMS Microstructures
  • 07:11Results: MinDE Self-Organization on Supported Lipid Bilayers
  • 07:44Conclusion

Summary

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我们提供了一个协议,在体外自我组织的精神和矿山的研究, 在支持的脂质双层在开放室。此外, 我们还描述了如何将该检测包裹在脂质包覆的 microcompartments 中, 通过反应约束模拟体内条件。

Tags

Keywords: In Vitro ReconstitutionSelf-organizing Protein PatternsSupported Lipid BilayersGeometric CuesPattern FormationMultilamellar VesiclesFreeze-thawLipid ExtruderSulfuric AcidHydrogen PeroxideCoverslipsSupported Lipid Bilayer FormationHeat Block

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