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应用造血干细胞移植评估骨髓增生异常综合征的起源
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
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应用造血干细胞移植评估骨髓增生异常综合征的起源

DOI:
10.3791/58140-v

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06:39 min

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October 03, 2018

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, , ,
1Genetics Branch,Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2LGI Flow Cytometry Core Facility,Center for Cancer Research, National Cancer Institute, National Institutes of Health

Capitoli

  • 00:04Titolo
  • 00:39Recipient Mouse Preparation and Bone Marrow Nucleated Cell (BMNC) Harvest
  • 02:25BMNC Fluorescence-activated Cell Sorting (FACS) and Hematopoietic Stem Cell Transplantation (HSCT)
  • 04:52Results: Representative HSCT Analyses
  • 06:08Conclusion

Summary

Traduzione automatica

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我们描述了利用造血干细胞移植 (HSCT) 来评估基因工程造血细胞的恶性潜能。HSCT 可用于评估体内各种恶性造血细胞, 并产生大量小鼠骨髓增生异常综合征 (MDS) 或白血病, 以评估新的治疗方法。

Tags

Keywords: Hematopoietic Stem Cell TransplantationMyelodysplastic SyndromeCancer Stem CellsFunctional AnalysisDisease Initiating CellsAdoptive TransferTotal Body IrradiationBone Marrow Nucleated CellsFlow SortingBiotinylated Anti-lineage AntibodyAPC Conjugated Anti-Sca1 Antibody

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