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Acellular and Cellular Lung Model to Study Tumor Metastasis
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JoVE Journal Ricerca sul cancro
Acellular and Cellular Lung Model to Study Tumor Metastasis

Acellular and Cellular Lung Model to Study Tumor Metastasis

DOI:
10.3791/58145-v

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08:31 min

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August 19, 2018

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1Department of Surgery,Houston Methodist Hospital Research Institute, 2Division of Thoracic Surgery, Department of Surgery,Weill Cornell Medical College, Houston Methodist Hospital

Capitoli

  • 00:04Titolo
  • 00:48Rat Lung Harvest
  • 02:16Lung Decellularization
  • 03:28Bioreactor Setup
  • 05:43Metastasis Model and Cell Seeding
  • 07:05Results: Representative Images of the Ex Vivo 4-D Lung Model for Metastasis
  • 08:02Conclusion

Summary

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Here, we present a protocol for an ex vivo lung cancer model that mimics the steps of tumor progression and helps to isolate a primary tumor, circulating tumor cells, and metastatic lesions.

Tags

Acellular Lung ModelCellular Lung ModelTumor MetastasisCancer ProgressionCirculating Tumor CellsMetastatic LesionsPersonalized TherapyTherapeutic DrugsImmune CellsRatThoracic CavityThoracotomyHeparinPBSTracheaAortaHeart-lung BlockPulmonary ArteryDecellularizationSodium Dodecyl SulfateNon-ionic Detergent

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