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透明质酸基水凝胶对3维患者源性胶质瘤细胞培养的研究

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Bioingegneria

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JoVE Journal Bioingegneria

Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

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透明质酸基水凝胶对3维患者源性胶质瘤细胞培养的研究

DOI:

10.3791/58176-v

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08:35 min

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August 24, 2018

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Weikun Xiao, Arshia Ehsanipour, Alireza Sohrabi, Stephanie K. Seidlits

¹Department of Bioengineering,University of California, Los Angeles, ²Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute,University of California, Los Angeles

Capitoli

00:04Titolo
00:31Hydrogel Crosslinking and Cell Encapsulation
03:11Hydrogel Culture Single Cell Extraction for Flow Cytometry and Cryopreservation for Sectioning
06:10Results: Hyaluronic Acid-Based 3D Patient-Derived Glioblastoma Cell Hydrogel Cultures
07:55Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个协议, 三维培养病人的胶质瘤细胞在正交可调谐生物材料设计, 以模仿大脑基质。这种方法提供了一种体外实验平台, 它能维持体内胶质瘤细胞的许多特征, 通常在培养中丢失。

Tags

Keywords: Hyaluronic Acid Hydrogels 3D Cell Culture Glioblastoma Extracellular Matrix Patient-derived Cells Cell Encapsulation PEG-Mal Silicone Rubber Molds Cell Dissociation Cell Suspension Gelation

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