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Quantifying Acute Changes in Renal Sympathetic Nerve Activity in Response to Central Nervous System Manipulations in Anesthetized Rats
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Neuroscienze
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JoVE Journal Neuroscienze
Quantifying Acute Changes in Renal Sympathetic Nerve Activity in Response to Central Nervous System Manipulations in Anesthetized Rats

Quantifying Acute Changes in Renal Sympathetic Nerve Activity in Response to Central Nervous System Manipulations in Anesthetized Rats

DOI:
10.3791/58205-v

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06:30 min

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September 11, 2018

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,
1Department of Biobehavioral Health Science, College of Nursing,University of Illinois at Chicago, 2Department of Anesthesiology,Medical College of Wisconsin and Zablocki VA Medical Center

Capitoli

  • 00:04Titolo
  • 00:36Femoral Artery Cannulation for Map Arterial Pressure (MAP) Monitoring
  • 01:41Brain Access, Renal Sympathetic Nerve Isolation, and Recording Quality Assessment
  • 05:02Results: Representative RSNA and Blood Pressure Responses to IV Phenylephrine Injection
  • 05:30Conclusion

Summary

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Methods for measuring sympathetic and cardiovascular responses to central nervous system (CNS) manipulations are important for advancing neuroscience. This protocol was developed to assist scientists with measuring and quantifying acute changes in renal sympathetic nerve activity (RSNA) in anesthetized rats (non-survival).

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Renal Sympathetic Nerve ActivityCentral Nervous SystemAnesthetized RatsAutonomic Nervous SystemArterial CannulationTelemetry ImplantBrain AccessStereotaxic SurgeryRSNA Electrode

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