A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

Anja Baufeld; Jens Vanselow

doi:10.3791/58208

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Biologia

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A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

DOI:

10.3791/58208-v

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05:36 min

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September 06, 2018

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Anja Baufeld, Jens Vanselow

¹Institute of Reproductive Biology,Leibniz Institute for Farm Animal Biology (FBN)

Capitoli

00:04Titolo
00:34Isolation of Bovine Granulosa Cells (GC)
01:37Cryopreservation of Cells
02:56Cell Culture
03:53Results: Analysis of Culture Bovine Granulosa Cells Under Various Conditions
04:56Conclusion

Summary

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A long-term culture model of bovine granulosa cells under serum-free conditions is described. This model allows researchers to study the effects of diverse factors and conditions as different plating densities on the characteristics of estrogen-producing bovine granulosa cells.

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

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