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Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

DOI:
10.3791/58210-v

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08:27 min

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September 20, 2018

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, , , , , , , ,
1Department of Medicine 1,University of Erlangen-Nuremberg

Capitoli

  • 00:04Titolo
  • 00:45Capillary and Cell Preparation
  • 03:54Live Cell Imaging of Dynamic Adhesion and Evaluation
  • 06:52Results: Representative Human PBMC Adherence Assessment
  • 07:33Conclusion

Summary

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Dynamic adhesion of immune cells to the vessel wall is a prerequisite for gut homing. Here, we present a protocol for a functional in vitro assay for the impact analysis of anti-integrin antibodies, chemokines or other factors on the dynamic cell adhesion of human cells using addressin-coated capillaries.

Tags

Dynamic Adhesion AssayAnti-adhesion TherapiesInflammatory Bowel DiseaseCell AdhesionFlow ConditionsAddressinPeripheral Blood Mononuclear CellsCell Tracking Dye

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