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Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons
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Neuroscienze
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JoVE Journal Neuroscienze
Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons

Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons

DOI:
10.3791/58229-v

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06:23 min

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October 30, 2018

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1Division of Neuromedical Science, Institute of Natural Medicine,University of Toyama

Capitoli

  • 00:04Titolo
  • 00:35Neuron Culture
  • 02:34Growth Cone Collapse Assay
  • 04:27Results: Aß1-42-induced Axonal Growth Cone Collapse
  • 05:41Conclusion

Summary

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Here a protocol to investigate the early effects of amyloid-β (Aβ) in the brain is presented. This shows that Aβ induces clathrin-mediated endocytosis and collapse of axonal growth cones. The protocol is useful in studying early effects of Aβ on axonal growth cones and may facilitate prevention of Alzheimer's disease.

Tags

Axonal Growth ConeAmyloid BetaAlzheimer’s DiseaseNeuron CultureEmbryonic MouseTrypsinDNAseSoybean Trypsin InhibitorCell FiltrationHemocytometerCell DensityAggregated Amyloid Beta 1-42

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