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无细胞爪卵萃取物微乳液细胞周期振荡的重建

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

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无细胞爪卵萃取物微乳液细胞周期振荡的重建

DOI:

10.3791/58240-v

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06:31 min

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September 27, 2018

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Ye Guan², Shiyuan Wang, Minjun Jin, Haotian Xu, Qiong Yang⁴

¹Department of Biophysics,University of Michigan, Ann Arbor, ²Department of Chemistry,University of Michigan, Ann Arbor, ³Department of Computer Science,Wayne State University, ⁴Department of Physics,University of Michigan, Ann Arbor

Capitoli

00:04Titolo
00:41Preparation of Cycling Xenopus Extracts
03:39Droplet Generation and Imaging
05:19Results: Cell-cycle System Capable of Reconstructing Mitotic Oscillator of Cdk1 and APC/C
05:53Conclusion

Summary

Traduzione automatica

本文提出了在油中微乳液中对爪蟾卵提取物进行单细胞水平的体外自持续有丝分裂振荡的生成方法。

Tags

Keywords: Cell-cycle Oscillations Xenopus Egg Extracts Microemulsions Cell-free System Mitotic Network Cell Cycle Clock High-throughput Quantitative Manipulation Cell Activation Cell Lysis Cell Extract Preparation

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