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基于网模的无支架三维心脏组织创建方法

JoVE Journal
Bioingegneria
Author Produced

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JoVE Journal Bioingegneria

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

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基于网模的无支架三维心脏组织创建方法

DOI:

10.3791/58252-v

•

06:57 min

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August 05, 2018

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Yang Bai², Enoch Yeung, Cecillia Lui, Chin Siang Ong, Isaree Pitaktong, Chenyu Huang, Takahiro Inoue, Hiroshi Matsushita, Chunye Ma, Narutoshi Hibino

¹Department of Cardiac Surgery,The First Hospital of Jilin University, ²Division of Cardiac Surgery,Johns Hopkins Hospital

Capitoli

00:00Titolo
00:14Process Flow of Cardiac Tissue Creation by Novel Mold
01:21Spheroids Creation by Hanging Drop Method
03:26Seeding Spheroids into the Mold Cavity
05:16Result: The 3D Cardiac Patch Contains of Stacked Spheroids with Functional Beating after Removing from Novel Mold
05:58Conclusion

Summary

Traduzione automatica

本协议描述了一种基于净模的方法来创建三维无支架的心脏组织, 结构完整性和同步跳动行为令人满意。

Tags

Scaffold-free Spheroids Hanging Drop System Mold-based Method Cardiomyocytes Fibroblasts Endothelial Cells Reusable Device Spontaneous Formation Mechanical Integrity

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