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En netto skimmel-baseret metode til stillads-gratis tre-dimensionelle hjerte væv skabelse
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Bioingegneria
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JoVE Journal Bioingegneria
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

En netto skimmel-baseret metode til stillads-gratis tre-dimensionelle hjerte væv skabelse

DOI:
10.3791/58252-v

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06:57 min

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August 05, 2018

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, , , , , , , , ,
1Department of Cardiac Surgery,The First Hospital of Jilin University, 2Division of Cardiac Surgery,Johns Hopkins Hospital

Capitoli

  • 00:00Titolo
  • 00:14Process Flow of Cardiac Tissue Creation by Novel Mold
  • 01:21Spheroids Creation by Hanging Drop Method
  • 03:26Seeding Spheroids into the Mold Cavity
  • 05:16Result: The 3D Cardiac Patch Contains of Stacked Spheroids with Functional Beating after Removing from Novel Mold
  • 05:58Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol beskriver en netto skimmel-baseret metode for at oprette tre-dimensionelle stillads-fri hjerte væv med tilfredsstillende strukturelle integritet og synkron slå adfærd.

Tags

Scaffold-freeSpheroidsHanging Drop SystemMold-based MethodCardiomyocytesFibroblastsEndothelial CellsReusable DeviceSpontaneous FormationMechanical Integrity

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