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人性化的 nod/scid/il2γ空 (hu-nsg) 小鼠 hiv 复制和延迟研究模型

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies

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人性化的 nod/scid/il2γ空 (hu-nsg) 小鼠 hiv 复制和延迟研究模型

DOI:

10.3791/58255-v

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07:10 min

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January 07, 2019

•

Xin Xia, Haitang Li, Sangeetha Satheesan², Jiehua Zhou, John J. Rossi

¹Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, ²Irell and Manela Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Capitoli

00:04Titolo
00:41Intrahepatic Hematopoietic Stem Cell (HSC) Injection
01:59HSC Engraftment Validation
03:48HIV Infection Analysis and Viral Suppression and Rebound Validation
05:20Results: Engraftment Validation and HIV Infection and Suppression Analyses
06:26Conclusion

Summary

Traduzione automatica

该方案提供了一种方法, 建立人性化小鼠 (hu-nsg) 通过肝内注射人类造血干细胞到辐射条件下的新生儿 nsg 小鼠。hu-nsg 小鼠容易感染 hiv 感染和组合抗逆转录病毒疗法 (cart), 是治疗艾滋病毒复制和潜伏期调查的合适病理生理模型。

Tags

Hu-NSG Mouse Model HIV Replication HIV Latency Non-invasive Human Stem Cells CD34+ HSCs NOD/SCID/IL2rÎ³null Mice CART HIV Infection Engraftment Validation Flow Cytometry

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