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Humaniseret SCID-NOD-IL2rγnull (hu-NSG) mus Model for HIV replikation og Latency undersøgelser
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Humaniseret SCID-NOD-IL2rγnull (hu-NSG) mus Model for HIV replikation og Latency undersøgelser

DOI:
10.3791/58255-v

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07:10 min

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January 07, 2019

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, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Irell and Manela Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Capitoli

  • 00:04Titolo
  • 00:41Intrahepatic Hematopoietic Stem Cell (HSC) Injection
  • 01:59HSC Engraftment Validation
  • 03:48HIV Infection Analysis and Viral Suppression and Rebound Validation
  • 05:20Results: Engraftment Validation and HIV Infection and Suppression Analyses
  • 06:26Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol indeholder en metode til at etablere humaniseret mus (hu-NSG) via intrahepatisk injektion af menneskelige hæmatopoietisk stamceller i stråling-aircondition neonatal NSG mus. Hu-NSG musen er modtagelige for HIV-infektion og kombinatorisk antiretroviral behandling (vogn) og tjener som en passende patofysiologiske model for HIV replikation og latency undersøgelser.

Tags

Keywords: Hu-NSG Mouse ModelHIV ReplicationHIV LatencyNon-invasiveHuman Stem CellsCD34+ HSCsNOD/SCID/IL2rγnull MiceCARTHIV InfectionEngraftment ValidationFlow Cytometry

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