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A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9
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Capitoli
- 00:04Titolo
- 00:43High-throughput Luciferase-based Proteolysis Assay
- 02:50Transfection (Day 1)
- 04:32Assay (Day 2)
- 06:41Results: Alterations in PCSK9 Proteolytic Activity are Common in Clinical Populations
- 07:52Conclusion
This protocol presents a method to evaluate the proteolytic activity of an intrinsically low-activity, single turnover protease in a cellular context. Specifically, this method is applied to evaluate the proteolytic activity of PCSK9, a key driver of lipid metabolism whose proteolytic activity is required for its ultimate hypercholesterolemic function.
