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高通量荧光素酶法评价单周转蛋白酶 PCSK9 的蛋白质水解

JoVE Journal
Biochimica

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JoVE Journal Biochimica

A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

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高通量荧光素酶法评价单周转蛋白酶 PCSK9 的蛋白质水解

DOI:

10.3791/58265-v

•

08:14 min

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August 28, 2018

•

John S. Chorba, Adri M. Galvan, Kevan M. Shokat

¹Division of Cardiology, Department of Medicine,Zuckerberg San Francisco General and University of California San Francisco, ²Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute,University of California San Francisco

Capitoli

00:04Titolo
00:43High-throughput Luciferase-based Proteolysis Assay
02:50Transfection (Day 1)
04:32Assay (Day 2)
06:41Results: Alterations in PCSK9 Proteolytic Activity are Common in Clinical Populations
07:52Conclusion

Summary

Traduzione automatica

本协议提出了一种评估细胞内一种内在的低活性、单周转蛋白酶的蛋白水解活性的方法。具体来说, 该方法用于评价 PCSK9 的蛋白水解活性, 这是脂代谢的关键驱动力, 其蛋白水解活性是其最终胆固醇功能所必需的。

Tags

Keywords: PCSK9 Proteolysis Luciferase Assay High-throughput Atherosclerotic Heart Disease Protease HEK293T Cells Transfection Cell Culture Biochemical System

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