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一种检测慢性淋巴细胞白血病新 nfat2 靶基因的染色质免疫沉淀法
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A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
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一种检测慢性淋巴细胞白血病新 nfat2 靶基因的染色质免疫沉淀法

DOI:
10.3791/58270-v

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09:52 min

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December 04, 2018

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, , , , , , , , ,
1Dept. of Hematology, Oncology and Immunology,University of Tübingen, 2Dept. of Endocrinology, Diabetology, Clinical Pathology and Metabolism,University of Tübingen

Capitoli

  • 00:04Titolo
  • 00:41Fixation, Cell Lysis, and Chromatin Shearing
  • 03:54Chromatin Immunoprecipitation
  • 08:31Results: qRT-PCR with Precipitated DNA Identified Specific Binding of NFAT2 to the LCK Promotor Region
  • 09:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

慢性淋巴细胞白血病 (cll) 是西方世界最常见的白血病。nfat 转录因子是多种细胞类型发育和激活的重要调节剂。在这里, 我们提出了一个在人的 cll 细胞中使用染色质免疫沉淀 (chip) 来识别 nfat2 的新靶向基因的协议。

Tags

Chromatin ImmunoprecipitationNFAT2Chronic Lymphocytic LeukemiaTranscription FactorsTarget GenesFixationShearingCell LysisSonicationProtease Inhibitor

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