Rivista
/
Ricerca sul cancro
/
Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation
JoVE Journal
Ricerca sul cancro
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation

Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation

DOI:
10.3791/58291-v

•

15:04 min

•

January 19, 2019

•

, , , , ,
1Centre de Recherche en Cancérologie et Immunologie Nantes Angers (CRCINA), Institut national de la santé et de la recherche médicale (INSERM), Université d’Angers, Université de Nantes, Nantes, France, 2Service de chirurgie pédiatrique,Centre hospitalier universitaire (CHU) de Nantes, Nantes, France, 3Unité de Formation et Recherche (UFR) des Sciences Pharmaceutiques et Biologiques,Université de Nantes, Nantes, France

Capitoli

  • 00:00Titolo
  • 01:38Evaluation of the Drug Interaction between mAb 8B6 and Topotecan in vitro
  • 07:35Generation of Human IMR5 Neuroblastoma Xenografts in NSG Mice
  • 12:18Representative Results
  • 14:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes how to assess synergism between an anticancer antibody and antineoplastic drugs in preclinical models by using the combination index equation of Chou and Talalay.

Tags

Anticancer AntibodyAntineoplastic DrugsCombination IndexSynergismImmunotherapyChemotherapyMonoclonal AntibodyNeuroblastomaTopotecanIn VitroIn VivoCell CultureXenograft Model

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/58291?article_type=v

Fluorescent Orthotopic Mouse Model of Pancreatic Cancer
check_url/it/58291?article_type=v

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles
check_url/it/58291?article_type=v

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
check_url/it/58291?article_type=v

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
check_url/it/58291?article_type=v

Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts
check_url/it/58291?article_type=v

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
check_url/it/58291?article_type=v

Detection of Protease Activity by Fluorescent Peptide Zymography
check_url/it/58291?article_type=v

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
check_url/it/58291?article_type=v

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR
check_url/it/58291?article_type=v

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Read Article